Fachhochschule Technikum Wien: Optical Biosensors / Nanotechnologies

Abstract: The course provides an introduction to optical biosensor technologies for rapid detection of molecular and biological species and for biomolecular interaction analysis. It comprises brief overview of optics fundamentals, technologies used for fabrication of optical micro and nano-structures, most common configurations of optical biosensors and their implementation to functional devices. The outline of the course of the following:


  • Definition of biosensor for detection of chemical and biological species. Overview of transducer technologies based on amperometric, acoustic, and optical measurements. Key performance characteristics of biosensors including sensitivity, detection range, and limit of detection. Application areas including medical diagnostics and drug development.
  • Introduction to optical phenomena exploited in optical biosensors: ray, wave optics, electromagnetic optics, total internal reflection, guided wave optics, surface plasmon resonance, fluorescence, and Raman scattering. 
  • echnologies for preparation of optical micro- and nano-structures: thin film deposition, top down and bottom up fabrications, photo- electron beam lithography, colloidal lithography, nano-imprint lithography.
  • Optical spectroscopy-based biosensors. Colorimetric, fluorescence, Raman, and IR-based detection. Sensor signal enhancement strategies - surface-enhanced Raman spectroscopy (SERS), surface-enhanced fluorescence (SEF).
  • Label-free optical biosensors. Localized and propagating surface plasmon resonance (SPR). Dielectric ring resonators and integrated optical interferometers. 
  • Heterogeneous assay-based biosensors. Reaction kinetics and evaluation of affinity binding constants for molecular interaction analysis (BIA).


Supporting materials: 

Slides in pdf can be obtained here: part1 and part2.